Detection of urovirulence factors in Escherichia coli by multiplex polymerase chain reaction
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چکیده
منابع مشابه
Detection of the eaeA Gene in Escherichia coli Isolated from Broiler Chickens by Polymerase Chain Reaction
The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in Escherichia coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs (lung, liver and spleen) were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. Escherichiacoliwas isolated from ...
متن کاملCharacterizing avian Escherichia coli isolates with multiplex polymerase chain reaction.
Colibacillosis caused by Escherichia coli infections account for significant morbidity and mortality in the poultry industry. Yet, despite the importance of colibacillosis, much about the virulence mechanisms employed by avian E. coli remains unknown. In recent years several genes have been linked to avian E. coli virulence, many of which reside on a large transmissible plasmid. In the present ...
متن کاملdetection of the eaea gene in escherichia coli isolated from broiler chickens by polymerase chain reaction
the aim of this study was to isolate escherichia coli from chickens and to determine the presence of the eaea gene, a virulence factor detected in escherichia coli, in the isolates by polymerase chain reaction (pcr). different chicken organs (lung, liver and spleen) were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. escherichiacoliwas isolated from ...
متن کاملGenotypic study of verocytotoxic Escherichia coli isolates from deer by multiplex polymerase chain reaction
AIM This study was planned to study the genotypes of verocytotoxigenic Escherichia coli (VTEC) in fecal samples of deer due to its public health significance. MATERIALS AND METHODS A total of 160 fecal samples of deer were taken from Mathura district and Kanpur Zoo and screened for VTEC genes by polymerase chain reaction (PCR). RESULTS All fecal samples were positive for E. coli. All the E....
متن کاملDetection of alpha-thalassemias by multiplex polymerase chain reaction.
Although alpha-thalassemia is the most common genetic abnormality in the world, there is currently no routine laboratory method to definitively identify individuals who are affected. We describe a rapid and simple method that utilizes deletion-sensitive primers to amplify normal DNA sequences. Deletions involving the regions responsible for most of the alpha-thalassemia cases in the US prevent ...
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ژورنال
عنوان ژورنال: FEMS Immunology and Medical Microbiology
سال: 1995
ISSN: 0928-8244
DOI: 10.1016/0928-8244(95)00053-a